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Servicebio Inc rankl primary and secondary antibodies
The effect of Shengu granules on intestinal barrier, FOXP3 cells <t>and</t> <t>OPG/RANKL</t> Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.
Rankl Primary And Secondary Antibodies, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis"

Article Title: Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2024.1320500

The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.
Figure Legend Snippet: The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.

Techniques Used: Expressing, Immunofluorescence

Related Articles

Incubation:

Article Title: Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis
Article Snippet: Total protein samples extracted from colon tissue were incubated with ZO-1 (Servicebio Technology Ltd., China) and Occludin (Servicebio Technology Ltd., China) primary and secondary antibodies, and total protein samples extracted from femur tissue were incubated with OPG (Servicebio Technology Ltd., China) and RANKL (Servicebio Technology Ltd., China) primary and secondary antibodies.

Expressing:

Article Title: Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis
Article Snippet: Total protein samples extracted from colon tissue were incubated with ZO-1 (Servicebio Technology Ltd., China) and Occludin (Servicebio Technology Ltd., China) primary and secondary antibodies, and total protein samples extracted from femur tissue were incubated with OPG (Servicebio Technology Ltd., China) and RANKL (Servicebio Technology Ltd., China) primary and secondary antibodies.

Immunofluorescence:

Article Title: Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis
Article Snippet: Total protein samples extracted from colon tissue were incubated with ZO-1 (Servicebio Technology Ltd., China) and Occludin (Servicebio Technology Ltd., China) primary and secondary antibodies, and total protein samples extracted from femur tissue were incubated with OPG (Servicebio Technology Ltd., China) and RANKL (Servicebio Technology Ltd., China) primary and secondary antibodies.



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Servicebio Inc rankl primary and secondary antibodies
The effect of Shengu granules on intestinal barrier, FOXP3 cells <t>and</t> <t>OPG/RANKL</t> Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.
Rankl Primary And Secondary Antibodies, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rankl+primary+and+secondary+antibodies/rankl+primary+and+secondary+antibodies/pmc10959285-95-42-43
Average 90 stars, based on 1 article reviews
rankl primary and secondary antibodies - by Bioz Stars, 2026-09
90/100 stars
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The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.

Journal: Frontiers in Microbiology

Article Title: Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis

doi: 10.3389/fmicb.2024.1320500

Figure Lengend Snippet: The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.

Article Snippet: Total protein samples extracted from colon tissue were incubated with ZO-1 (Servicebio Technology Ltd., China) and Occludin (Servicebio Technology Ltd., China) primary and secondary antibodies, and total protein samples extracted from femur tissue were incubated with OPG (Servicebio Technology Ltd., China) and RANKL (Servicebio Technology Ltd., China) primary and secondary antibodies.

Techniques: Expressing, Immunofluorescence